Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression

Swarna Ganesh, Krishnan Venkatakrishnan () and Bo Tan
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Swarna Ganesh: Institute for Biomedical Engineering, Science and Technology (I BEST), Partnership between Ryerson University and St. Michael’s Hospital
Krishnan Venkatakrishnan: Ryerson University
Bo Tan: Ryerson University

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract Cancer stem cells (CSC) can be identified by modifications in their genomic DNA. Here, we report a concept of precisely shrinking an organic semiconductor surface-enhanced Raman scattering (SERS) probe to quantum size, for investigating the epigenetic profile of CSC. The probe is used for tag-free genomic DNA detection, an approach towards the advancement of single-molecule DNA detection. The sensor detected structural, molecular and gene expression aberrations of genomic DNA in femtomolar concentration simultaneously in a single test. In addition to pointing out the divergences in genomic DNA of cancerous and non-cancerous cells, the quantum scale organic semiconductor was able to trace the expression of two genes which are frequently used as CSC markers. The quantum scale organic semiconductor holds the potential to be a new tool for label-free, ultra-sensitive multiplexed genomic analysis.

Date: 2020
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DOI: 10.1038/s41467-020-14774-3

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