Visualization and molecular characterization of whole-brain vascular networks with capillary resolution

Takeyuki Miyawaki (), Shota Morikawa, Etsuo A. Susaki, Ai Nakashima, Haruki Takeuchi, Shun Yamaguchi, Hiroki R. Ueda and Yuji Ikegaya
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Takeyuki Miyawaki: The University of Tokyo
Shota Morikawa: The University of Tokyo
Etsuo A. Susaki: The University of Tokyo
Ai Nakashima: The University of Tokyo
Haruki Takeuchi: The University of Tokyo
Shun Yamaguchi: Gifu University
Hiroki R. Ueda: The University of Tokyo
Yuji Ikegaya: The University of Tokyo

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Structural elucidation and molecular scrutiny of cerebral vasculature is crucial for understanding the functions and diseases of the brain. Here, we introduce SeeNet, a method for near-complete three-dimensional visualization of cerebral vascular networks with high signal-to-noise ratios compatible with molecular phenotyping. SeeNet employs perfusion of a multifunctional crosslinker, vascular casting by temperature-controlled polymerization of hybrid hydrogels, and a bile salt-based tissue-clearing technique optimized for observation of vascular connectivity. SeeNet is capable of whole-brain visualization of molecularly characterized cerebral vasculatures at the single-microvessel level. Moreover, SeeNet reveals a hitherto unidentified vascular pathway bridging cerebral and hippocampal vessels, thus serving as a potential tool to evaluate the connectivity of cerebral vasculature.

Date: 2020
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DOI: 10.1038/s41467-020-14786-z

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