The role of Mediator and Little Elongation Complex in transcription termination

Hidehisa Takahashi (), Amol Ranjan, Shiyuan Chen, Hidefumi Suzuki, Mio Shibata, Tomonori Hirose, Hiroko Hirose, Kazunori Sasaki, Ryota Abe, Kai Chen, Yanfeng He, Ying Zhang, Ichigaku Takigawa, Tadasuke Tsukiyama, Masashi Watanabe, Satoshi Fujii, Midori Iida, Junichi Yamamoto, Yuki Yamaguchi, Yutaka Suzuki, Masaki Matsumoto, Keiichi I. Nakayama, Michael P. Washburn, Anita Saraf, Laurence Florens, Shigeo Sato, Chieri Tomomori-Sato, Ronald C. Conaway, Joan W. Conaway () and Shigetsugu Hatakeyama ()
Additional contact information
Hidehisa Takahashi: Yokohama City University Graduate School of Medical Science
Amol Ranjan: Stowers Institute for Medical Research
Shiyuan Chen: Stowers Institute for Medical Research
Hidefumi Suzuki: Yokohama City University Graduate School of Medical Science
Mio Shibata: Hokkaido University
Tomonori Hirose: Yokohama City University Graduate School of Medical Science
Hiroko Hirose: Yokohama City University Graduate School of Medical Science
Kazunori Sasaki: Yokohama City University Graduate School of Medical Science
Ryota Abe: Yokohama City University Graduate School of Medical Science
Kai Chen: Stowers Institute for Medical Research
Yanfeng He: Stowers Institute for Medical Research
Ying Zhang: Stowers Institute for Medical Research
Ichigaku Takigawa: Hokkaido University
Tadasuke Tsukiyama: Hokkaido University
Masashi Watanabe: Hokkaido University
Satoshi Fujii: Kyushu Institute of Technology
Midori Iida: Kyushu Institute of Technology
Junichi Yamamoto: Department of Nanoparticle Translational Research Tokyo Medical University
Yuki Yamaguchi: Tokyo Institute of Technology
Yutaka Suzuki: The University of Tokyo
Masaki Matsumoto: Kyushu University
Keiichi I. Nakayama: Kyushu University
Michael P. Washburn: Stowers Institute for Medical Research
Anita Saraf: Stowers Institute for Medical Research
Laurence Florens: Stowers Institute for Medical Research
Shigeo Sato: Stowers Institute for Medical Research
Chieri Tomomori-Sato: Stowers Institute for Medical Research
Ronald C. Conaway: Stowers Institute for Medical Research
Joan W. Conaway: Stowers Institute for Medical Research
Shigetsugu Hatakeyama: Hokkaido University

Nature Communications, 2020, vol. 11, issue 1, 1-20

Abstract: Abstract Mediator is a coregulatory complex that regulates transcription of Pol II-dependent genes. Previously, we showed that human Mediator subunit MED26 plays a role in the recruitment of Super Elongation Complex (SEC) or Little Elongation Complex (LEC) to regulate the expression of certain genes. MED26 plays a role in recruiting SEC to protein-coding genes including c-myc and LEC to small nuclear RNA (snRNA) genes. However, how MED26 engages SEC or LEC to regulate distinct genes is unclear. Here, we provide evidence that MED26 recruits LEC to modulate transcription termination of non-polyadenylated transcripts including snRNAs and mRNAs encoding replication-dependent histone (RDH) at Cajal bodies. Our findings indicate that LEC recruited by MED26 promotes efficient transcription termination by Pol II through interaction with CBC-ARS2 and NELF/DSIF, and promotes 3′ end processing by enhancing recruitment of Integrator or Heat Labile Factor to snRNA or RDH genes, respectively.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14849-1

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DOI: 10.1038/s41467-020-14849-1

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