Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma

Bin Kong, Yun Chen, Rui Liu, Xi Liu, Changyong Liu, Zengwu Shao, Liming Xiong, Xianning Liu, Wei Sun () and Shengli Mi ()
Additional contact information
Bin Kong: Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute
Yun Chen: Tsinghua Shenzhen International Graduate School
Rui Liu: Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School
Xi Liu: Beijing Children’s Hospital
Changyong Liu: Shenzhen University
Zengwu Shao: Huazhong University Science & Technology
Liming Xiong: Huazhong University Science & Technology
Xianning Liu: Shaanxi Institute of Ophthalmology
Wei Sun: Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute
Shengli Mi: Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract Regeneration of corneal stroma has always been a challenge due to its sophisticated structure and keratocyte-fibroblast transformation. In this study, we fabricate grid poly (ε-caprolactone)-poly (ethylene glycol) microfibrous scaffold and infuse the scaffold with gelatin methacrylate (GelMA) hydrogel to obtain a 3 D fiber hydrogel construct; the fiber spacing is adjusted to fabricate optimal construct that simulates the stromal structure with properties most similar to the native cornea. The topological structure (3 D fiber hydrogel, 3 D GelMA hydrogel, and 2 D culture dish) and chemical factors (serum, ascorbic acid, insulin, and β-FGF) are examined to study their effects on the differentiation of limbal stromal stem cells to keratocytes or fibroblasts and the phenotype maintenance, in vitro and in vivo tissue regeneration. The results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environment for the maintenance of keratocyte phenotype and the regeneration of damaged corneal stroma.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-14887-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14887-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-14887-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().