Grasp55−/− mice display impaired fat absorption and resistance to high-fat diet-induced obesity

Jiyoon Kim, Hyeyon Kim, Shin Hye Noh, Dong Geon Jang, Shi-Young Park, Dongkook Min, Hyunki Kim, Hee-Seok Kweon, Hoguen Kim, Sowon Aum, Sookyung Seo, Cheol Soo Choi, Hail Kim, Jae Woo Kim, Seok Jun Moon, Heon Yung Gee and Min Goo Lee ()
Additional contact information
Jiyoon Kim: Yonsei University College of Medicine
Hyeyon Kim: Yonsei University College of Dentistry
Shin Hye Noh: Yonsei University College of Medicine
Dong Geon Jang: Yonsei University College of Medicine
Shi-Young Park: Gachon University College of Medicine
Dongkook Min: Yonsei University College of Medicine
Hyunki Kim: Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology
Hee-Seok Kweon: Center for Research Equipment, Korea Basic Science Institute
Hoguen Kim: Yonsei University College of Medicine
Sowon Aum: Yonsei University College of Medicine
Sookyung Seo: Yonsei University College of Medicine
Cheol Soo Choi: Gachon University College of Medicine
Hail Kim: Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology
Jae Woo Kim: Yonsei University College of Medicine
Seok Jun Moon: Yonsei University College of Dentistry
Heon Yung Gee: Yonsei University College of Medicine
Min Goo Lee: Yonsei University College of Medicine

Nature Communications, 2020, vol. 11, issue 1, 1-17

Abstract: Abstract The Golgi apparatus plays a central role in the intracellular transport of macromolecules. However, molecular mechanisms of Golgi-mediated lipid transport remain poorly understood. Here, we show that genetic inactivation of the Golgi-resident protein GRASP55 in mice reduces whole-body fat mass via impaired intestinal fat absorption and evokes resistance to high-fat diet induced body weight gain. Mechanistic analyses reveal that GRASP55 participates in the Golgi-mediated lipid droplet (LD) targeting of some LD-associated lipases, such as ATGL and MGL, which is required for sustained lipid supply for chylomicron assembly and secretion. Consequently, GRASP55 deficiency leads to reduced chylomicron secretion and abnormally large LD formation in intestinal epithelial cells upon exogenous lipid challenge. Notably, deletion of dGrasp in Drosophila causes similar defects of lipid accumulation in the midgut. These results highlight the importance of the Golgi complex in cellular lipid regulation, which is evolutionary conserved, and uncover potential therapeutic targets for obesity-associated diseases.

Date: 2020
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DOI: 10.1038/s41467-020-14912-x

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