Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells

Magdalena Wagner, Masahito Yoshihara, Iyadh Douagi, Anastasios Damdimopoulos, Sarita Panula, Sophie Petropoulos, Haojiang Lu, Karin Pettersson, Kerstin Palm, Shintaro Katayama, Outi Hovatta, Juha Kere, Fredrik Lanner () and Pauliina Damdimopoulou ()
Additional contact information
Magdalena Wagner: Karolinska Institutet and Karolinska University Hospital
Masahito Yoshihara: Karolinska Institutet
Iyadh Douagi: Karolinska Institutet
Anastasios Damdimopoulos: Bioinformatics and Expression Analysis core facility, Karolinska Institutet
Sarita Panula: Karolinska Institutet and Karolinska University Hospital
Sophie Petropoulos: Karolinska Institutet and Karolinska University Hospital
Haojiang Lu: Karolinska Institutet and Karolinska University Hospital
Karin Pettersson: Karolinska Institutet and Karolinska University Hospital
Kerstin Palm: Karolinska Institutet and Karolinska University Hospital
Shintaro Katayama: Karolinska Institutet
Outi Hovatta: Karolinska Institutet and Karolinska University Hospital
Juha Kere: Karolinska Institutet
Fredrik Lanner: Karolinska Institutet and Karolinska University Hospital
Pauliina Damdimopoulou: Karolinska Institutet and Karolinska University Hospital

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. Here, we report single-cell transcriptomes and cell surface antigen profiles of over 24,000 cells from high quality ovarian cortex samples from 21 patients. Our data identify transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Cells captured by DDX4 antibody are perivascular cells, not oogonial stem cells. Our data do not support the existence of germline stem cells in adult human ovaries, thereby reinforcing the dogma of a limited ovarian reserve.

Date: 2020
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DOI: 10.1038/s41467-020-14936-3

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