Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

Colin W. Hiebert (), Matthew J. Moscou (), Tim Hewitt, Burkhard Steuernagel, Inma Hernández-Pinzón, Phon Green, Vincent Pujol, Peng Zhang, Matthew N. Rouse, Yue Jin, Robert A. McIntosh, Narayana Upadhyaya, Jianping Zhang, Sridhar Bhavani, Jan Vrána, Miroslava Karafiátová, Li Huang, Tom Fetch, Jaroslav Doležel, Brande B. H. Wulff, Evans Lagudah () and Wolfgang Spielmeyer ()
Additional contact information
Colin W. Hiebert: Agriculture and Agri-Food Canada, Morden Research and Development Centre
Matthew J. Moscou: University of East Anglia
Tim Hewitt: University of Sydney
Burkhard Steuernagel: John Innes Centre, Norwich Research Park
Inma Hernández-Pinzón: University of East Anglia
Phon Green: University of East Anglia
Vincent Pujol: The Australian National University
Peng Zhang: University of Sydney
Matthew N. Rouse: University of Minnesota
Yue Jin: University of Minnesota
Robert A. McIntosh: University of Sydney
Narayana Upadhyaya: CSIRO Agriculture & Food
Jianping Zhang: CSIRO Agriculture & Food
Sridhar Bhavani: CIMMYT, ICRAF House, United Nations Avenue
Jan Vrána: Centre of the Region Haná for Biotechnological and Agricultural Research
Miroslava Karafiátová: Centre of the Region Haná for Biotechnological and Agricultural Research
Li Huang: Montana State University
Tom Fetch: Agriculture and Agri-Food Canada, Morden Research and Development Centre
Jaroslav Doležel: Centre of the Region Haná for Biotechnological and Agricultural Research
Brande B. H. Wulff: John Innes Centre, Norwich Research Park
Evans Lagudah: CSIRO Agriculture & Food
Wolfgang Spielmeyer: CSIRO Agriculture & Food

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.

Date: 2020
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DOI: 10.1038/s41467-020-14937-2

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