Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

Oliver Xiaoou Dong, Shu Yu, Rashmi Jain, Nan Zhang, Phat Q. Duong, Corinne Butler, Yan Li, Anna Lipzen, Joel A. Martin, Kerrie W. Barry, Jeremy Schmutz, Li Tian and Pamela C. Ronald ()
Additional contact information
Oliver Xiaoou Dong: University of California
Shu Yu: University of California
Rashmi Jain: University of California
Nan Zhang: University of California
Phat Q. Duong: University of California
Corinne Butler: University of California
Yan Li: University of California
Anna Lipzen: Department of Energy Joint Genome Institute
Joel A. Martin: Department of Energy Joint Genome Institute
Kerrie W. Barry: Department of Energy Joint Genome Institute
Jeremy Schmutz: Department of Energy Joint Genome Institute
Li Tian: University of California
Pamela C. Ronald: University of California

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Targeted insertion of transgenes at pre-determined plant genomic safe harbors provides a desirable alternative to insertions at random sites achieved through conventional methods. Most existing cases of targeted gene insertion in plants have either relied on the presence of a selectable marker gene in the insertion cassette or occurred at low frequency with relatively small DNA fragments (

Date: 2020
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DOI: 10.1038/s41467-020-14981-y

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