An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity

Camilla Calandrini, Frans Schutgens, Rurika Oka, Thanasis Margaritis, Tito Candelli, Luka Mathijsen, Carola Ammerlaan, Ravian L. Ineveld, Sepide Derakhshan, Sanne Haan, Emmy Dolman, Philip Lijnzaad, Lars Custers, Harry Begthel, Hindrik H. D. Kerstens, Lindy L. Visser, Maarten Rookmaaker, Marianne Verhaar, Godelieve A. M. Tytgat, Patrick Kemmeren, Ronald R. Krijger, Reem Al-Saadi, Kathy Pritchard-Jones, Marcel Kool, Anne C. Rios, Marry M. Heuvel-Eibrink, Jan J. Molenaar, Ruben Boxtel, Frank C. P. Holstege, Hans Clevers and Jarno Drost ()
Additional contact information
Camilla Calandrini: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Frans Schutgens: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Rurika Oka: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Thanasis Margaritis: Princess Máxima Center for Pediatric Oncology
Tito Candelli: Princess Máxima Center for Pediatric Oncology
Luka Mathijsen: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Carola Ammerlaan: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Ravian L. Ineveld: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Sepide Derakhshan: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Sanne Haan: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Emmy Dolman: Princess Máxima Center for Pediatric Oncology
Philip Lijnzaad: Princess Máxima Center for Pediatric Oncology
Lars Custers: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Harry Begthel: Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center
Hindrik H. D. Kerstens: Princess Máxima Center for Pediatric Oncology
Lindy L. Visser: Princess Máxima Center for Pediatric Oncology
Maarten Rookmaaker: University Medical Center, Department of Nephrology and Hypertension
Marianne Verhaar: University Medical Center, Department of Nephrology and Hypertension
Godelieve A. M. Tytgat: Princess Máxima Center for Pediatric Oncology
Patrick Kemmeren: Princess Máxima Center for Pediatric Oncology
Ronald R. Krijger: Princess Máxima Center for Pediatric Oncology
Reem Al-Saadi: University College London, UCL Great Ormond Street Institute of Child Health
Kathy Pritchard-Jones: University College London, UCL Great Ormond Street Institute of Child Health
Marcel Kool: Princess Máxima Center for Pediatric Oncology
Anne C. Rios: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Marry M. Heuvel-Eibrink: Princess Máxima Center for Pediatric Oncology
Jan J. Molenaar: Princess Máxima Center for Pediatric Oncology
Ruben Boxtel: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Frank C. P. Holstege: Princess Máxima Center for Pediatric Oncology
Hans Clevers: Oncode Institute, Princess Máxima Center for Pediatric Oncology
Jarno Drost: Oncode Institute, Princess Máxima Center for Pediatric Oncology

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15155-6

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DOI: 10.1038/s41467-020-15155-6

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