In TFIIH the Arch domain of XPD is mechanistically essential for transcription and DNA repair

Stefan Peissert, Florian Sauer, Daniel B. Grabarczyk, Cathy Braun, Gudrun Sander, Arnaud Poterszman, Jean-Marc Egly (), Jochen Kuper () and Caroline Kisker ()
Additional contact information
Stefan Peissert: University of Würzburg
Florian Sauer: University of Würzburg
Daniel B. Grabarczyk: University of Würzburg
Cathy Braun: Institut de Génétique et de Biologie Moléculaire et Cellulaire Illkirch Cedex, C.U.
Gudrun Sander: University of Würzburg
Arnaud Poterszman: Institut de Génétique et de Biologie Moléculaire et Cellulaire Illkirch Cedex, C.U.
Jean-Marc Egly: Institut de Génétique et de Biologie Moléculaire et Cellulaire Illkirch Cedex, C.U.
Jochen Kuper: University of Würzburg
Caroline Kisker: University of Würzburg

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract The XPD helicase is a central component of the general transcription factor TFIIH which plays major roles in transcription and nucleotide excision repair (NER). Here we present the high-resolution crystal structure of the Arch domain of XPD with its interaction partner MAT1, a central component of the CDK activating kinase complex. The analysis of the interface led to the identification of amino acid residues that are crucial for the MAT1-XPD interaction. More importantly, mutagenesis of the Arch domain revealed that these residues are essential for the regulation of (i) NER activity by either impairing XPD helicase activity or the interaction of XPD with XPG; (ii) the phosphorylation of the RNA polymerase II and RNA synthesis. Our results reveal how MAT1 shields these functionally important residues thereby providing insights into how XPD is regulated by MAT1 and defining the Arch domain as a major mechanistic player within the XPD scaffold.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-15241-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15241-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-15241-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().