Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord

Jun Zhang, Gopalakrishnan Chandrasekaran, Wenting Li, Dong-Young Kim, In Young Jeong, So-Hyun Lee, Ting Liang, Jin Young Bae, Isaac Choi, Hyuno Kang, Jin-Soo Maeng, Myeong-Kyu Kim, Taewon Lee, Seung Woo Park, Min Jung Kim, Hyung-Seok Kim, Hyunju Ro, Yong Chul Bae, Hae-Chul Park, Eun Young Choi () and Seok-Yong Choi ()
Additional contact information
Jun Zhang: Chonnam National University Medical School
Gopalakrishnan Chandrasekaran: Chonnam National University Medical School
Wenting Li: Chonnam National University Medical School
Dong-Young Kim: University of Ulsan College of Medicine
In Young Jeong: Korea University
So-Hyun Lee: Chonnam National University Medical School
Ting Liang: Chonnam National University Medical School
Jin Young Bae: School of Dentistry, Kyungpook National University
Isaac Choi: College of Bioscience and Biotechnology, Chungnam National University
Hyuno Kang: Korea Basic Science Institute
Jin-Soo Maeng: Korea Food Research Institute
Myeong-Kyu Kim: Chonnam National University Medical School
Taewon Lee: College of Science and Technology, Korea University
Seung Woo Park: Yonsei University College of Medicine
Min Jung Kim: Sookmyung Women’s University
Hyung-Seok Kim: Chonnam National University Medical School
Hyunju Ro: College of Bioscience and Biotechnology, Chungnam National University
Yong Chul Bae: School of Dentistry, Kyungpook National University
Hae-Chul Park: Korea University
Eun Young Choi: University of Ulsan College of Medicine
Seok-Yong Choi: Chonnam National University Medical School

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract Ependymal cells (ECs) are multiciliated neuroepithelial cells that line the ventricles of the brain and the central canal of the spinal cord (SC). How ependymal motile cilia are maintained remains largely unexplored. Here we show that zebrafish embryos deficient in Wnt signaling have defective motile cilia, yet harbor intact basal bodies. With respect to maintenance of ependymal motile cilia, plcδ3a is a target gene of Wnt signaling. Lack of Connexin43 (Cx43), especially its channel function, decreases motile cilia and intercellular Ca2+ wave (ICW) propagation. Genetic ablation of cx43 in zebrafish and mice diminished motile cilia. Finally, Cx43 is also expressed in ECs of the human SC. Taken together, our findings indicate that gap junction mediated ICWs play an important role in the maintenance of ependymal motile cilia, and suggest that the enhancement of functional gap junctions by pharmacological or genetic manipulations may be adopted to ameliorate motile ciliopathy.

Date: 2020
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DOI: 10.1038/s41467-020-15248-2

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