Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach

Alexis Theodorou, Evelina Liarou, David M. Haddleton, Iren Georgia Stavrakaki, Panagiotis Skordalidis, Richard Whitfield, Athina Anastasaki () and Kelly Velonia ()
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Alexis Theodorou: University of Crete
Evelina Liarou: University of Warwick
David M. Haddleton: University of Warwick
Iren Georgia Stavrakaki: University of Crete
Panagiotis Skordalidis: University of Crete
Richard Whitfield: ETH Zurich
Athina Anastasaki: ETH Zurich
Kelly Velonia: University of Crete

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract The immense application potential of amphiphilic protein-polymer conjugates remains largely unexplored, as established “grafting from” synthetic protocols involve time-consuming, harsh and disruptive deoxygenation methods, while “grafting to” approaches result in low yields. Here we report an oxygen tolerant, photoinduced CRP approach which readily affords quantitative yields of protein-polymer conjugates within 2 h, avoiding damage to the secondary structure of the protein and providing easily accessible means to produce biomacromolecular assemblies. Importantly, our methodology is compatible with multiple proteins (e.g. BSA, HSA, GOx, beta-galactosidase) and monomer classes including acrylates, methacrylates, styrenics and acrylamides. The polymerizations are conveniently conducted in plastic syringes and in the absence of any additives or external deoxygenation procedures using low-organic content media and ppm levels of copper. The robustness of the protocol is further exemplified by its implementation under UV, blue light or even sunlight irradiation as well as in buffer, nanopure, tap or even sea water.

Date: 2020
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DOI: 10.1038/s41467-020-15259-z

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