Optogenetic restoration of retinal ganglion cell activity in the living primate

Juliette E. McGregor (), Tyler Godat, Kamal R. Dhakal, Keith Parkins, Jennifer M. Strazzeri, Brittany A. Bateman, William S. Fischer, David R. Williams and William H. Merigan ()
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Juliette E. McGregor: University of Rochester
Tyler Godat: University of Rochester
Kamal R. Dhakal: University of Rochester
Keith Parkins: University of Rochester
Jennifer M. Strazzeri: University of Rochester
Brittany A. Bateman: University of Rochester Medical Center
William S. Fischer: University of Rochester
David R. Williams: University of Rochester
William H. Merigan: University of Rochester

Nature Communications, 2020, vol. 11, issue 1, 1-9

Abstract: Abstract Optogenetic therapies for vision restoration aim to confer intrinsic light sensitivity to retinal ganglion cells when photoreceptors have degenerated and light sensitivity has been irreversibly lost. We combine adaptive optics ophthalmoscopy with calcium imaging to optically record optogenetically restored retinal ganglion cell activity in the fovea of the living primate. Recording from the intact eye of a living animal, we compare the patterns of activity evoked by the optogenetic actuator ChrimsonR with natural photoreceptor mediated stimulation in the same retinal ganglion cells. Optogenetic responses are recorded more than one year following administration of the therapy and two weeks after acute loss of photoreceptor input in the living animal. This in vivo imaging approach could be paired with any therapy to minimize the number of primates required to evaluate restored activity on the retinal level, while maximizing translational benefit by using an appropriate pre-clinical model of the human visual system.

Date: 2020
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DOI: 10.1038/s41467-020-15317-6

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