Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells

Alvaro Plaza Reyes, Sandra Petrus-Reurer, Sara Padrell Sánchez, Pankaj Kumar, Iyadh Douagi, Hammurabi Bartuma, Monica Aronsson, Sofie Westman, Emma Lardner, Helder André, Anna Falk, Emeline F. Nandrot, Anders Kvanta and Fredrik Lanner ()
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Alvaro Plaza Reyes: Karolinska Institutet
Sandra Petrus-Reurer: Karolinska Institutet
Sara Padrell Sánchez: Karolinska Institutet
Pankaj Kumar: Karolinska Institutet
Iyadh Douagi: Karolinska Institutet
Hammurabi Bartuma: Karolinska Institutet
Monica Aronsson: Karolinska Institutet
Sofie Westman: Karolinska Institutet
Emma Lardner: Karolinska Institutet
Helder André: Karolinska Institutet
Anna Falk: Karolinska Institutet
Emeline F. Nandrot: Sorbonne Université
Anders Kvanta: Karolinska Institutet
Fredrik Lanner: Karolinska Institutet

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of age-related macular degeneration. Although the inherent pigmentation of the RPE cells have been useful to grossly evaluate differentiation efficiency and allowed manual isolation of pigmented structures, accurate quantification and automated isolation has been challenging. To address this issue, here we perform a comprehensive antibody screening and identify cell surface markers for RPE cells. We show that these markers can be used to isolate RPE cells during in vitro differentiation and to track, quantify and improve differentiation efficiency. Finally, these surface markers aided to develop a robust, direct and scalable monolayer differentiation protocol on human recombinant laminin-111 and −521 without the need for manual isolation.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15326-5

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DOI: 10.1038/s41467-020-15326-5

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