Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions

Antonio Tedeschi (), Jorge Almagro, Matthew J. Renshaw, Hendrik A. Messal, Axel Behrens and Mark Petronczki
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Antonio Tedeschi: The Francis Crick Institute
Jorge Almagro: The Francis Crick Institute
Matthew J. Renshaw: The Francis Crick Institute
Hendrik A. Messal: The Francis Crick Institute
Axel Behrens: The Francis Crick Institute
Mark Petronczki: Cancer Research UK London Research Institute

Nature Communications, 2020, vol. 11, issue 1, 1-16

Abstract: Abstract In mammalian cell lines, the endosomal sorting complex required for transport (ESCRT)-III mediates abscission, the process that physically separates daughter cells and completes cell division. Cep55 protein is regarded as the master regulator of abscission, because it recruits ESCRT-III to the midbody (MB), the site of abscission. However, the importance of this mechanism in a mammalian organism has never been tested. Here we show that Cep55 is dispensable for mouse embryonic development and adult tissue homeostasis. Cep55-knockout offspring show microcephaly and primary neural progenitors require Cep55 and ESCRT for survival and abscission. However, Cep55 is dispensable for cell division in embryonic or adult tissues. In vitro, division of primary fibroblasts occurs without Cep55 and ESCRT-III at the midbody and is not affected by ESCRT depletion. Our work defines Cep55 as an abscission regulator only in specific tissue contexts and necessitates the re-evaluation of an alternative ESCRT-independent cell division mechanism.

Date: 2020
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DOI: 10.1038/s41467-020-15359-w

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