Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts

Julia Grimmer, Stefan Helm, Dirk Dobritzsch, Gerd Hause, Gerta Shema, René P. Zahedi and Sacha Baginsky ()
Additional contact information
Julia Grimmer: Martin-Luther-University Halle-Wittenberg
Stefan Helm: Martin-Luther-University Halle-Wittenberg
Dirk Dobritzsch: Martin-Luther-University Halle-Wittenberg
Gerd Hause: Biocenter of the University, Martin-Luther-University Halle-Wittenberg
Gerta Shema: Leibniz-Institut für Analytische Wissenschaften -ISAS- e.V.
René P. Zahedi: Leibniz-Institut für Analytische Wissenschaften -ISAS- e.V.
Sacha Baginsky: Martin-Luther-University Halle-Wittenberg

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract The proteasome is an essential protein-degradation machinery in eukaryotic cells that controls protein turnover and thereby the biogenesis and function of cell organelles. Chloroplasts import thousands of nuclear-encoded precursor proteins from the cytosol, suggesting that the bulk of plastid proteins is transiently exposed to the cytosolic proteasome complex. Therefore, there is a cytosolic equilibrium between chloroplast precursor protein import and proteasomal degradation. We show here that a shift in this equilibrium, induced by mild genetic proteasome impairment, results in elevated precursor protein abundance in the cytosol and significantly increased accumulation of functional photosynthetic complexes in protein import-deficient chloroplasts. Importantly, a proteasome lid mutant shows improved photosynthetic performance, even in the absence of an import defect, signifying that functional precursors are continuously degraded. Hence, turnover of plastid precursors in the cytosol represents a mechanism to constrain thylakoid membrane assembly and photosynthetic electron transport.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-15539-8 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15539-8

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-15539-8

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().