Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages

Jennifer M. Rosenbluth, Ron C. J. Schackmann, G. Kenneth Gray, Laura M. Selfors, Carman Man-Chung Li, Mackenzie Boedicker, Hendrik J. Kuiken, Andrea Richardson, Jane Brock, Judy Garber, Deborah Dillon, Norman Sachs, Hans Clevers and Joan S. Brugge ()
Additional contact information
Jennifer M. Rosenbluth: Harvard Medical School
Ron C. J. Schackmann: Harvard Medical School
G. Kenneth Gray: Harvard Medical School
Laura M. Selfors: Harvard Medical School
Carman Man-Chung Li: Harvard Medical School
Mackenzie Boedicker: Harvard Medical School
Hendrik J. Kuiken: Harvard Medical School
Andrea Richardson: Brigham & Women’s Hospital
Jane Brock: Brigham & Women’s Hospital
Judy Garber: Dana-Farber Cancer Institute
Deborah Dillon: Brigham & Women’s Hospital
Norman Sachs: Hubrecht Institute
Hans Clevers: Hubrecht Institute
Joan S. Brugge: Harvard Medical School

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Basal/stem and luminal progenitor cells can differentiate in culture to generate mature basal and luminal cell types, including ER+ cells that have been challenging to maintain in culture. Cells associated with increased cancer risk can also be propagated. Single-cell analyses of matched organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution representation of the multiple mammary epithelial cell types in the organoids, and demonstrate that protein expression patterns of the tissue of origin can be preserved in culture. These studies indicate that organoid cultures provide a valuable platform for studies of mammary differentiation, transformation, and breast cancer risk.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-15548-7 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15548-7

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-15548-7

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().