Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

Xiuyuan Ou, Yan Liu, Xiaobo Lei, Pei Li, Dan Mi, Lili Ren, Li Guo, Ruixuan Guo, Ting Chen, Jiaxin Hu, Zichun Xiang, Zhixia Mu, Xing Chen, Jieyong Chen, Keping Hu, Qi Jin (), Jianwei Wang () and Zhaohui Qian ()
Additional contact information
Xiuyuan Ou: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Yan Liu: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Xiaobo Lei: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Pei Li: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Dan Mi: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Lili Ren: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Li Guo: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Ruixuan Guo: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Ting Chen: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Jiaxin Hu: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Zichun Xiang: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Zhixia Mu: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Xing Chen: Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical Collage (PUMC)
Jieyong Chen: Hengshui Third People’s Hospital
Keping Hu: Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical Collage (PUMC)
Qi Jin: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Jianwei Wang: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College
Zhaohui Qian: Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract Since 2002, beta coronaviruses (CoV) have caused three zoonotic outbreaks, SARS-CoV in 2002–2003, MERS-CoV in 2012, and the newly emerged SARS-CoV-2 in late 2019. However, little is currently known about the biology of SARS-CoV-2. Here, using SARS-CoV-2 S protein pseudovirus system, we confirm that human angiotensin converting enzyme 2 (hACE2) is the receptor for SARS-CoV-2, find that SARS-CoV-2 enters 293/hACE2 cells mainly through endocytosis, that PIKfyve, TPC2, and cathepsin L are critical for entry, and that SARS-CoV-2 S protein is less stable than SARS-CoV S. Polyclonal anti-SARS S1 antibodies T62 inhibit entry of SARS-CoV S but not SARS-CoV-2 S pseudovirions. Further studies using recovered SARS and COVID-19 patients’ sera show limited cross-neutralization, suggesting that recovery from one infection might not protect against the other. Our results present potential targets for development of drugs and vaccines for SARS-CoV-2.

Date: 2020
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DOI: 10.1038/s41467-020-15562-9

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