Nitrogen limitation reveals large reserves in metabolic and translational capacities of yeast

Rosemary Yu, Kate Campbell, Rui Pereira, Johan Björkeroth, Qi Qi, Egor Vorontsov, Carina Sihlbom and Jens Nielsen ()
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Rosemary Yu: Chalmers University of Technology
Kate Campbell: Chalmers University of Technology
Rui Pereira: Chalmers University of Technology
Johan Björkeroth: Chalmers University of Technology
Qi Qi: Chalmers University of Technology
Egor Vorontsov: University of Gothenburg
Carina Sihlbom: University of Gothenburg
Jens Nielsen: Chalmers University of Technology

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwise reducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and transcript content. Combining multi-omics analysis with metabolic modeling, we find that seven metabolic superpathways maintain >50% metabolic capacity in reserve, with glucose metabolism maintaining >80% reserve capacity. Cells maintain >50% reserve in translational capacity for 2490 out of 3361 expressed genes (74%), with a disproportionately large reserve dedicated to translating metabolic proteins. Finally, ribosome reserves contain up to 30% sub-stoichiometric ribosomal proteins, with activation of reserve translational capacity associated with selective upregulation of 17 ribosomal proteins. Together, our dataset provides a quantitative link between yeast physiology and cellular economics, which could be leveraged in future cell engineering through targeted proteome streamlining.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15749-0

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DOI: 10.1038/s41467-020-15749-0

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