Real-time non-contact cellular imaging and angiography of human cornea and limbus with common-path full-field/SD OCT

Viacheslav Mazlin (), Peng Xiao (), Jules Scholler, Kristina Irsch, Kate Grieve, Mathias Fink and A. Claude Boccara ()
Additional contact information
Viacheslav Mazlin: PSL University, CNRS
Peng Xiao: PSL University, CNRS
Jules Scholler: PSL University, CNRS
Kristina Irsch: Sorbonne University, UMR_S 968/INSERM, U968/CNRS, UMR_7210
Kate Grieve: Sorbonne University, UMR_S 968/INSERM, U968/CNRS, UMR_7210
Mathias Fink: PSL University, CNRS
A. Claude Boccara: PSL University, CNRS

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract In today’s clinics, a cell-resolution view of the cornea can be achieved only with a confocal microscope (IVCM) in contact with the eye. Here, we present a common-path full-field/spectral-domain OCT microscope (FF/SD OCT), which enables cell-detail imaging of the entire ocular surface in humans (central and peripheral cornea, limbus, sclera, tear film) without contact and in real-time. Real-time performance is achieved through rapid axial eye tracking and simultaneous defocusing correction. Images contain cells and nerves, which can be quantified over a millimetric field-of-view, beyond the capability of IVCM and conventional OCT. In the limbus, palisades of Vogt, vessels, and blood flow can be resolved with high contrast without contrast agent injection. The fast imaging speed of 275 frames/s (0.6 billion pixels/s) allows direct monitoring of blood flow dynamics, enabling creation of high-resolution velocity maps. Tear flow velocity and evaporation time can be measured without fluorescein administration.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-15792-x Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15792-x

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-15792-x

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().