High-throughput cell and spheroid mechanics in virtual fluidic channels

Muzaffar H. Panhwar, Fabian Czerwinski, Venkata A. S. Dabbiru, Yesaswini Komaragiri, Bob Fregin, Doreen Biedenweg, Peter Nestler, Ricardo H. Pires and Oliver Otto ()
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Muzaffar H. Panhwar: Universität Greifswald
Fabian Czerwinski: Universität Greifswald
Venkata A. S. Dabbiru: Universität Greifswald
Yesaswini Komaragiri: Universität Greifswald
Bob Fregin: Universität Greifswald
Doreen Biedenweg: Universitätsmedizin Greifswald
Peter Nestler: Universität Greifswald
Ricardo H. Pires: Universität Greifswald
Oliver Otto: Universität Greifswald

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract Microfluidics by soft lithography has proven to be of key importance for biophysics and life science research. While being based on replicating structures of a master mold using benchtop devices, design modifications are time consuming and require sophisticated cleanroom equipment. Here, we introduce virtual fluidic channels as a flexible and robust alternative to microfluidic devices made by soft lithography. Virtual channels are liquid-bound fluidic systems that can be created in glass cuvettes and tailored in three dimensions within seconds for rheological studies on a wide size range of biological samples. We demonstrate that the liquid-liquid interface imposes a hydrodynamic stress on confined samples, and the resulting strain can be used to calculate rheological parameters from simple linear models. In proof-of-principle experiments, we perform high-throughput rheology inside a flow cytometer cuvette and show the Young’s modulus of isolated cells exceeds the one of the corresponding tissue by one order of magnitude.

Date: 2020
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DOI: 10.1038/s41467-020-15813-9

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