Comprehensive aptamer-based screening identifies a spectrum of urinary biomarkers of lupus nephritis across ethnicities

Samantha Stanley, Kamala Vanarsa, Samar Soliman, Deena Habazi, Claudia Pedroza, Gabriel Gidley, Ting Zhang, Shree Mohan, Evan Der, Hemant Suryawanshi, Thomas Tuschl, Jill Buyon, Chaim Putterman, Chi Chiu Mok, Michelle Petri, Ramesh Saxena and Chandra Mohan ()
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Samantha Stanley: University of Houston
Kamala Vanarsa: University of Houston
Samar Soliman: University of Houston
Deena Habazi: University of Houston
Claudia Pedroza: University of Texas Health Science Center at Houston
Gabriel Gidley: University of Houston
Ting Zhang: University of Houston
Shree Mohan: University of Houston
Evan Der: Albert Einstein College of Medicine
Hemant Suryawanshi: Rockefeller University
Thomas Tuschl: Rockefeller University
Jill Buyon: New York University
Chaim Putterman: Albert Einstein College of Medicine
Chi Chiu Mok: Tuen Mun Hospital
Michelle Petri: Johns Hopkins University School of Medicine
Ramesh Saxena: University of Texas Southwestern Medical Center
Chandra Mohan: University of Houston

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract Emerging urinary biomarkers continue to show promise in evaluating lupus nephritis (LN). Here, we screen urine from active LN patients for 1129 proteins using an aptamer-based platform, followed by ELISA validation in two independent cohorts comprised of 127 inactive lupus, 107 active LN, 67 active non-renal lupus patients and 74 healthy controls, of three different ethnicities. Urine proteins that best distinguish active LN from inactive disease are ALCAM, PF-4, properdin, and VCAM-1 among African-Americans, sE-selectin, VCAM-1, BFL-1 and Hemopexin among Caucasians, and ALCAM, VCAM-1, TFPI and PF-4 among Asians. Most of these correlate significantly with disease activity indices in the respective ethnic groups, and surpass conventional metrics in identifying active LN, with better sensitivity, and negative/positive predictive values. Several elevated urinary molecules are also expressed within the kidneys in LN, based on single-cell RNAseq analysis. Longitudinal studies are warranted to assess the utility of these biomarkers in tracking lupus nephritis.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15986-3

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DOI: 10.1038/s41467-020-15986-3

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