Light-powered Escherichia coli cell division for chemical production

Qiang Ding, Danlei Ma, Gao-Qiang Liu, Yang Li, Liang Guo, Cong Gao, Guipeng Hu, Chao Ye, Jia Liu, Liming Liu and Xiulai Chen ()
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Qiang Ding: Jiangnan University
Danlei Ma: Jiangnan University
Gao-Qiang Liu: Central South University of Forestry and Technology
Yang Li: Jiangnan University
Liang Guo: Jiangnan University
Cong Gao: Jiangnan University
Guipeng Hu: Jiangnan University
Chao Ye: Jiangnan University
Jia Liu: Jiangnan University
Liming Liu: Jiangnan University
Xiulai Chen: Jiangnan University

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm−1 and acetoin titer to 67.2 g·L−1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 μm3 and poly(lactate-co-3-hydroxybutyrate) titer to 14.31 g·L−1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories.

Date: 2020
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DOI: 10.1038/s41467-020-16154-3

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