hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma

Pauline Herviou, Morgane Le Bras, Leïla Dumas, Corinne Hieblot, Julia Gilhodes, Gianluca Cioci, Jean-Philippe Hugnot, Alfred Ameadan, François Guillonneau, Erik Dassi (), Anne Cammas () and Stefania Millevoi ()
Additional contact information
Pauline Herviou: Cancer Research Center of Toulouse (CRCT), INSERM UMR 1037
Morgane Le Bras: Cancer Research Center of Toulouse (CRCT), INSERM UMR 1037
Leïla Dumas: Cancer Research Center of Toulouse (CRCT), INSERM UMR 1037
Corinne Hieblot: Cancer Research Center of Toulouse (CRCT), INSERM UMR 1037
Julia Gilhodes: Institut Universitaire du Cancer de Toulouse-Oncopole
Gianluca Cioci: TBI, Université de Toulouse, CNRS, INRA, INSA
Jean-Philippe Hugnot: Université de Montpellier 2
Alfred Ameadan: Université de Paris, Inserm U1016-institut Cochin, Labex GReX
François Guillonneau: Université de Paris, Inserm U1016-institut Cochin, Labex GReX
Erik Dassi: University of Trento Via Sommarive 9
Anne Cammas: Cancer Research Center of Toulouse (CRCT), INSERM UMR 1037
Stefania Millevoi: Cancer Research Center of Toulouse (CRCT), INSERM UMR 1037

Nature Communications, 2020, vol. 11, issue 1, 1-17

Abstract: Abstract RNA G-quadruplexes (RG4s) are four-stranded structures known to control mRNA translation of cancer relevant genes. RG4 formation is pervasive in vitro but not in cellulo, indicating the existence of poorly characterized molecular machinery that remodels RG4s and maintains them unfolded. Here, we performed a quantitative proteomic screen to identify cytosolic proteins that interact with a canonical RG4 in its folded and unfolded conformation. Our results identified hnRNP H/F as important components of the cytoplasmic machinery modulating the structural integrity of RG4s, revealed their function in RG4-mediated translation and uncovered the underlying molecular mechanism impacting the cellular stress response linked to the outcome of glioblastoma.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-16168-x Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16168-x

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-16168-x

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().