Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

K. K. Khoo, I. Galleano, F. Gasparri, R. Wieneke, H. Harms, M. H. Poulsen, H. C. Chua, M. Wulf, R. Tampé and S. A. Pless ()
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K. K. Khoo: University of Copenhagen
I. Galleano: University of Copenhagen
F. Gasparri: University of Copenhagen
R. Wieneke: Goethe University Frankfurt
H. Harms: University of Copenhagen
M. H. Poulsen: University of Copenhagen
H. C. Chua: University of Copenhagen
M. Wulf: University of Copenhagen
R. Tampé: Goethe University Frankfurt
S. A. Pless: University of Copenhagen

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods have limitations in the number and type of modifications that can be introduced, especially in live cells. Here, we present an approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of existing protein enigineering methods.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16208-6

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DOI: 10.1038/s41467-020-16208-6

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