Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation

Aurélie Bertin (), Nicola Franceschi (), Eugenio Mora, Sourav Maity, Maryam Alqabandi, Nolwen Miguet, Aurélie Cicco, Wouter H. Roos, Stéphanie Mangenot, Winfried Weissenhorn () and Patricia Bassereau ()
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Aurélie Bertin: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168
Nicola Franceschi: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168
Eugenio Mora: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168
Sourav Maity: Moleculaire Biofysica, Zernike Instituut, Rijksuniversiteit Groningen, Nijenborgh 4
Maryam Alqabandi: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168
Nolwen Miguet: Univ. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale (IBS), 71, avenue des Martyrs
Aurélie Cicco: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168
Wouter H. Roos: Moleculaire Biofysica, Zernike Instituut, Rijksuniversiteit Groningen, Nijenborgh 4
Stéphanie Mangenot: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168
Winfried Weissenhorn: Univ. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale (IBS), 71, avenue des Martyrs
Patricia Bassereau: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract Endosomal sorting complexes for transport-III (ESCRT-III) assemble in vivo onto membranes with negative Gaussian curvature. How membrane shape influences ESCRT-III polymerization and how ESCRT-III shapes membranes is yet unclear. Human core ESCRT-III proteins, CHMP4B, CHMP2A, CHMP2B and CHMP3 are used to address this issue in vitro by combining membrane nanotube pulling experiments, cryo-electron tomography and AFM. We show that CHMP4B filaments preferentially bind to flat membranes or to tubes with positive mean curvature. Both CHMP2B and CHMP2A/CHMP3 assemble on positively curved membrane tubes. Combinations of CHMP4B/CHMP2B and CHMP4B/CHMP2A/CHMP3 are recruited to the neck of pulled membrane tubes and reshape vesicles into helical “corkscrew-like” membrane tubes. Sub-tomogram averaging reveals that the ESCRT-III filaments assemble parallel and locally perpendicular to the tube axis, highlighting the mechanical stresses imposed by ESCRT-III. Our results underline the versatile membrane remodeling activity of ESCRT-III that may be a general feature required for cellular membrane remodeling processes.

Date: 2020
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DOI: 10.1038/s41467-020-16368-5

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