A SAM-I riboswitch with the ability to sense and respond to uncharged initiator tRNA

Dong-Jie Tang, Xinyu Du, Qiang Shi, Jian-Ling Zhang, Yuan-Ping He, Yan-Miao Chen, Zhenhua Ming, Dan Wang, Wan-Ying Zhong, Yu-Wei Liang, Jin-Yang Liu, Jian-Ming Huang, Yun-Shi Zhong, Shi-Qi An, Hongzhou Gu () and Ji-Liang Tang ()
Additional contact information
Dong-Jie Tang: Guangxi University
Xinyu Du: Fudan University
Qiang Shi: Zhongshan Hospital of Fudan University
Jian-Ling Zhang: Guangxi University
Yuan-Ping He: Guangxi University
Yan-Miao Chen: Guangxi University
Zhenhua Ming: Guangxi University
Dan Wang: Guangxi University
Wan-Ying Zhong: Guangxi University
Yu-Wei Liang: Guangxi University
Jin-Yang Liu: Guangxi University
Jian-Ming Huang: Fudan University Pudong Medical Center
Yun-Shi Zhong: Zhongshan Hospital of Fudan University
Shi-Qi An: University of Southampton
Hongzhou Gu: Fudan University
Ji-Liang Tang: Guangxi University

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract All known riboswitches use their aptamer to senese one metabolite signal and their expression platform to regulate gene expression. Here, we characterize a SAM-I riboswitch (SAM-IXcc) from the Xanthomonas campestris that regulates methionine synthesis via the met operon. In vitro and in vivo experiments show that SAM-IXcc controls the met operon primarily at the translational level in response to cellular S-adenosylmethionine (SAM) levels. Biochemical and genetic data demonstrate that SAM-IXcc expression platform not only can repress gene expression in response to SAM binding to SAM-IXcc aptamer but also can sense and bind uncharged initiator Met tRNA, resulting in the sequestering of the anti-Shine-Dalgarno (SD) sequence and freeing the SD for translation initiation. These findings identify a SAM-I riboswitch with a dual functioning expression platform that regulates methionine synthesis through a previously unrecognized mechanism and discover a natural tRNA-sensing RNA element. This SAM-I riboswitch appears to be highly conserved in Xanthomonas species.

Date: 2020
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DOI: 10.1038/s41467-020-16417-z

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