Pushing the boundaries of optoacoustic microscopy by total impulse response characterization

Markus Seeger, Dominik Soliman, Juan Aguirre, Gael Diot, Jakob Wierzbowski and Vasilis Ntziachristos ()
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Markus Seeger: Technical University of Munich
Dominik Soliman: Technical University of Munich
Juan Aguirre: Technical University of Munich
Gael Diot: Technical University of Munich
Jakob Wierzbowski: Technical University of Munich
Vasilis Ntziachristos: Technical University of Munich

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract Optical microscopy improves in resolution and signal-to-noise ratio by correcting for the system’s point spread function; a measure of how a point source is resolved, typically determined by imaging nanospheres. Optical-resolution optoacoustic (photoacoustic) microscopy could be similarly corrected, especially to account for the spatially-dependent signal distortions induced by the acoustic detection and the time-resolved and bi-polar nature of optoacoustic signals. Correction algorithms must therefore include the spatial dependence of signals’ origins and profiles in time, i.e. the four-dimensional total impulse response (TIR). However, such corrections have been so far impeded by a lack of efficient TIR-characterization methods. We introduce high-quality TIR determination based on spatially-distributed optoacoustic point sources (SOAPs), produced by scanning an optical focus on an axially-translatable 250 nm gold layer. Using a spatially-dependent TIR-correction improves the signal-to-noise ratio by >10 dB and the axial resolution by ~30%. This accomplishment displays a new performance paradigm for optoacoustic microscopy.

Date: 2020
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DOI: 10.1038/s41467-020-16565-2

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