Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients

Chek Meng Poh, Guillaume Carissimo, Bei Wang, Siti Naqiah Amrun, Cheryl Yi-Pin Lee, Rhonda Sin-Ling Chee, Siew-Wai Fong, Nicholas Kim-Wah Yeo, Wen-Hsin Lee, Anthony Torres-Ruesta, Yee-Sin Leo, Mark I-Cheng Chen, Seow-Yen Tan, Louis Yi Ann Chai, Shirin Kalimuddin, Shirley Seah Gek Kheng, Siew-Yee Thien, Barnaby Edward Young, David C. Lye, Brendon John Hanson, Cheng-I Wang, Laurent Renia and Lisa F. P. Ng ()
Additional contact information
Chek Meng Poh: Technology and Research, Immunos, Biopolis
Guillaume Carissimo: Technology and Research, Immunos, Biopolis
Bei Wang: Technology and Research, Immunos, Biopolis
Siti Naqiah Amrun: Technology and Research, Immunos, Biopolis
Cheryl Yi-Pin Lee: Technology and Research, Immunos, Biopolis
Rhonda Sin-Ling Chee: Technology and Research, Immunos, Biopolis
Siew-Wai Fong: Technology and Research, Immunos, Biopolis
Nicholas Kim-Wah Yeo: Technology and Research, Immunos, Biopolis
Wen-Hsin Lee: Technology and Research, Immunos, Biopolis
Anthony Torres-Ruesta: Technology and Research, Immunos, Biopolis
Yee-Sin Leo: 16 Jalan Tan Tock Seng
Mark I-Cheng Chen: 16 Jalan Tan Tock Seng
Seow-Yen Tan: Changi General Hospital
Louis Yi Ann Chai: Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng
Shirin Kalimuddin: Singapore General Hospital
Shirley Seah Gek Kheng: DSO National Laboratories
Siew-Yee Thien: Singapore General Hospital
Barnaby Edward Young: 16 Jalan Tan Tock Seng
David C. Lye: 16 Jalan Tan Tock Seng
Brendon John Hanson: DSO National Laboratories
Cheng-I Wang: Technology and Research, Immunos, Biopolis
Laurent Renia: Technology and Research, Immunos, Biopolis
Lisa F. P. Ng: Technology and Research, Immunos, Biopolis

Nature Communications, 2020, vol. 11, issue 1, 1-7

Abstract: Abstract Given the ongoing SARS-CoV-2 pandemic, identification of immunogenic targets against the coronavirus spike glycoprotein will provide crucial advances towards the development of sensitive diagnostic tools and potential vaccine candidate targets. In this study, using pools of overlapping linear B-cell peptides, we report two IgG immunodominant regions on SARS-CoV-2 spike glycoprotein that are recognised by sera from COVID-19 convalescent patients. Notably, one is specific to SARS-CoV-2, which is located in close proximity to the receptor binding domain. The other region, which is localised at the fusion peptide, could potentially function as a pan-SARS target. Functionally, antibody depletion assays demonstrate that antibodies targeting these immunodominant regions significantly alter virus neutralisation capacities. Taken together, identification and validation of these neutralising B-cell epitopes will provide insights towards the design of diagnostics and vaccine candidates against this high priority coronavirus.

Date: 2020
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DOI: 10.1038/s41467-020-16638-2

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