The characterization of Mediator 12 and 13 as conditional positive gene regulators in Arabidopsis

Qikun Liu (), Sylvain Bischof, C. Jake Harris, Zhenhui Zhong, Lingyu Zhan, Calvin Nguyen, Andrew Rashoff, William D. Barshop, Fei Sun, Suhua Feng, Magdalena Potok, Javier Gallego-Bartolome, Jixian Zhai, James A. Wohlschlegel, Michael F. Carey, Jeffrey A. Long and Steven E. Jacobsen ()
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Qikun Liu: Peking University
Sylvain Bischof: University of California at Los Angeles
C. Jake Harris: University of California at Los Angeles
Zhenhui Zhong: University of California at Los Angeles
Lingyu Zhan: University of California at Los Angeles
Calvin Nguyen: University of California at Los Angeles
Andrew Rashoff: University of California at Los Angeles
William D. Barshop: University of California at Los Angeles
Fei Sun: University of California at Los Angeles
Suhua Feng: University of California at Los Angeles
Magdalena Potok: University of California at Los Angeles
Javier Gallego-Bartolome: University of California at Los Angeles
Jixian Zhai: Southern University of Science and Technology
James A. Wohlschlegel: University of California at Los Angeles
Michael F. Carey: University of California at Los Angeles
Jeffrey A. Long: University of California at Los Angeles
Steven E. Jacobsen: University of California at Los Angeles

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract Mediator 12 (MED12) and MED13 are components of the Mediator multi-protein complex, that facilitates the initial steps of gene transcription. Here, in an Arabidopsis mutant screen, we identify MED12 and MED13 as positive gene regulators, both of which contribute broadly to morc1 de-repressed gene expression. Both MED12 and MED13 are preferentially required for the expression of genes depleted in active chromatin marks, a chromatin signature shared with morc1 re-activated loci. We further discover that MED12 tends to interact with genes that are responsive to environmental stimuli, including light and radiation. We demonstrate that light-induced transient gene expression depends on MED12, and is accompanied by a concomitant increase in MED12 enrichment during induction. In contrast, the steady-state expression level of these genes show little dependence on MED12, suggesting that MED12 is primarily required to aid the expression of genes in transition from less-active to more active states.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16651-5

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DOI: 10.1038/s41467-020-16651-5

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