Spitzenkörper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds

Peng Zheng, Tu Anh Nguyen, Jie Yun Wong, Michelle Lee, The-Anh Nguyen, Jing-Song Fan, Daiwen Yang and Gregory Jedd ()
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Peng Zheng: National University of Singapore
Tu Anh Nguyen: National University of Singapore
Jie Yun Wong: National University of Singapore
Michelle Lee: National University of Singapore
The-Anh Nguyen: National University of Singapore
Jing-Song Fan: National University of Singapore
Daiwen Yang: National University of Singapore
Gregory Jedd: National University of Singapore

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract The Spitzenkörper (SPK) constitutes a collection of secretory vesicles and polarity-related proteins intimately associated with polarized growth of fungal hyphae. Many SPK-localized proteins are known, but their assembly and dynamics remain poorly understood. Here, we identify protein-protein interaction cascades leading to assembly of two SPK scaffolds and recruitment of diverse effectors in Neurospora crassa. Both scaffolds are transported to the SPK by the myosin V motor (MYO-5), with the coiled-coil protein SPZ-1 acting as cargo adaptor. Neither scaffold appears to be required for accumulation of SPK secretory vesicles. One scaffold consists of Leashin-2 (LAH-2), which is required for SPK localization of the signalling kinase COT-1 and the glycolysis enzyme GPI-1. The other scaffold comprises a complex of Janus-1 (JNS-1) and the polarisome protein SPA-2. Via its Spa homology domain (SHD), SPA-2 recruits a calponin domain-containing F-actin effector (CCP-1). The SHD NMR structure reveals a conserved surface groove required for effector binding. Similarities between SPA-2/JNS-1 and the metazoan GIT/PIX complex identify foundational features of the cell polarity apparatus that predate the fungal-metazoan divergence.

Date: 2020
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DOI: 10.1038/s41467-020-16712-9

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