Structural basis for divergent C–H hydroxylation selectivity in two Rieske oxygenases

April L. Lukowski, Jianxin Liu, Jennifer Bridwell-Rabb () and Alison R. H. Narayan ()
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April L. Lukowski: University of Michigan
Jianxin Liu: University of Michigan
Jennifer Bridwell-Rabb: University of Michigan
Alison R. H. Narayan: University of Michigan

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Biocatalysts that perform C–H hydroxylation exhibit exceptional substrate specificity and site-selectivity, often through the use of high valent oxidants to activate these inert bonds. Rieske oxygenases are examples of enzymes with the ability to perform precise mono- or dioxygenation reactions on a variety of substrates. Understanding the structural features of Rieske oxygenases responsible for control over selectivity is essential to enable the development of this class of enzymes for biocatalytic applications. Decades of research has illuminated the critical features common to Rieske oxygenases, however, structural information for enzymes that functionalize diverse scaffolds is limited. Here, we report the structures of two Rieske monooxygenases involved in the biosynthesis of paralytic shellfish toxins (PSTs), SxtT and GxtA, adding to the short list of structurally characterized Rieske oxygenases. Based on these structures, substrate-bound structures, and mutagenesis experiments, we implicate specific residues in substrate positioning and the divergent reaction selectivity observed in these two enzymes.

Date: 2020
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DOI: 10.1038/s41467-020-16729-0

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