Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2

Chunqing Wang, Yongmei Li, Shuai Yan, Hao Wang, Xianfeng Shao, Mingming Xiao, Baicai Yang, Guoxuan Qin, Ruirui Kong, Ruibing Chen () and Ning Zhang ()
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Chunqing Wang: Tianjin Medical University
Yongmei Li: Tianjin Medical University
Shuai Yan: Tianjin Medical University
Hao Wang: Tianjin Medical University
Xianfeng Shao: Tianjin Medical University
Mingming Xiao: Tianjin Medical University
Baicai Yang: Tianjin Medical University
Guoxuan Qin: Tianjin University
Ruirui Kong: Peking University Health Science Center
Ruibing Chen: Tianjin University
Ning Zhang: Tianjin Medical University

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract Interacting with proteins is a crucial way for long noncoding RNAs (lncRNAs) to exert their biological responses. Here we report a high throughput strategy to characterize lncRNA interacting proteins in vivo by combining tobramycin affinity purification and mass spectrometric analysis (TOBAP-MS). Using this method, we identify 140 candidate binding proteins for lncRNA highly upregulated in liver cancer (HULC). Intriguingly, HULC directly binds to two glycolytic enzymes, lactate dehydrogenase A (LDHA) and pyruvate kinase M2 (PKM2). Mechanistic study suggests that HULC functions as an adaptor molecule that enhances the binding of LDHA and PKM2 to fibroblast growth factor receptor type 1 (FGFR1), leading to elevated phosphorylation of these two enzymes and consequently promoting glycolysis. This study provides a convenient method to study lncRNA interactome in vivo and reveals a unique mechanism by which HULC promotes Warburg effect by orchestrating the enzymatic activities of glycolytic enzymes.

Date: 2020
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DOI: 10.1038/s41467-020-16966-3

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