Long-term culture of human pancreatic slices as a model to study real-time islet regeneration

Mirza Muhammad Fahd Qadir, Silvia Álvarez-Cubela, Jonathan Weitz, Julia K. Panzer, Dagmar Klein, Yaisa Moreno-Hernández, Sirlene Cechin, Alejandro Tamayo, Joana Almaça, Helmut Hiller, Maria Beery, Irina Kusmartseva, Mark Atkinson, Stephan Speier, Camillo Ricordi, Alberto Pugliese, Alejandro Caicedo, Christopher A. Fraker, Ricardo Luis Pastori () and Juan Domínguez-Bendala ()
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Mirza Muhammad Fahd Qadir: University of Miami Miller School of Medicine
Silvia Álvarez-Cubela: University of Miami Miller School of Medicine
Jonathan Weitz: University of Miami Miller School of Medicine
Julia K. Panzer: University of Miami Miller School of Medicine
Dagmar Klein: University of Miami Miller School of Medicine
Yaisa Moreno-Hernández: University of Miami Miller School of Medicine
Sirlene Cechin: University of Miami Miller School of Medicine
Alejandro Tamayo: University of Miami Miller School of Medicine
Joana Almaça: University of Miami Miller School of Medicine
Helmut Hiller: University of Florida
Maria Beery: University of Florida
Irina Kusmartseva: University of Florida
Mark Atkinson: University of Florida
Stephan Speier: Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, Helmholtz Zentrum München
Camillo Ricordi: University of Miami Miller School of Medicine
Alberto Pugliese: University of Miami Miller School of Medicine
Alejandro Caicedo: University of Miami Miller School of Medicine
Christopher A. Fraker: University of Miami Miller School of Medicine
Ricardo Luis Pastori: University of Miami Miller School of Medicine
Juan Domínguez-Bendala: University of Miami Miller School of Medicine

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract The culture of live pancreatic tissue slices is a powerful tool for the interrogation of physiology and pathology in an in vitro setting that retains near-intact cytoarchitecture. However, current culture conditions for human pancreatic slices (HPSs) have only been tested for short-term applications, which are not permissive for the long-term, longitudinal study of pancreatic endocrine regeneration. Using a culture system designed to mimic the physiological oxygenation of the pancreas, we demonstrate high viability and preserved endocrine and exocrine function in HPS for at least 10 days after sectioning. This extended lifespan allowed us to dynamically lineage trace and quantify the formation of insulin-producing cells in HPS from both non-diabetic and type 2 diabetic donors. This technology is expected to be of great impact for the conduct of real-time regeneration/developmental studies in the human pancreas.

Date: 2020
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DOI: 10.1038/s41467-020-17040-8

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