Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins

Noura Alzahofi, Tobias Welz, Christopher L. Robinson, Emma L. Page, Deborah A. Briggs, Amy K. Stainthorp, James Reekes, David A. Elbe, Felix Straub, Wouter W. Kallemeijn, Edward W. Tate, Philip S. Goff, Elena V. Sviderskaya, Marta Cantero, Lluis Montoliu, Francois Nedelec, Amanda K. Miles, Maryse Bailly, Eugen Kerkhoff and Alistair N. Hume ()
Additional contact information
Noura Alzahofi: University of Nottingham
Tobias Welz: University Hospital Regensburg
Christopher L. Robinson: University of Nottingham
Emma L. Page: University of Nottingham
Deborah A. Briggs: University of Nottingham
Amy K. Stainthorp: University of Nottingham
James Reekes: University of Nottingham
David A. Elbe: University of Nottingham
Felix Straub: University Hospital Regensburg
Wouter W. Kallemeijn: Imperial College London, Molecular Sciences Research Hub
Edward W. Tate: Imperial College London, Molecular Sciences Research Hub
Philip S. Goff: St. George’s, University of London
Elena V. Sviderskaya: St. George’s, University of London
Marta Cantero: Centro Nacional de Biotecnologia (CNB-CSIC)
Lluis Montoliu: Centro Nacional de Biotecnologia (CNB-CSIC)
Francois Nedelec: Cambridge University
Amanda K. Miles: Nottingham Trent University
Maryse Bailly: UCL Institute of Ophthalmology
Eugen Kerkhoff: University Hospital Regensburg
Alistair N. Hume: University of Nottingham

Nature Communications, 2020, vol. 11, issue 1, 1-21

Abstract: Abstract Cell biologists generally consider that microtubules and actin play complementary roles in long- and short-distance transport in animal cells. On the contrary, using melanosomes of melanocytes as a model, we recently discovered that the motor protein myosin-Va works with dynamic actin tracks to drive long-range organelle dispersion in opposition to microtubules. This suggests that in animals, as in yeast and plants, myosin/actin can drive long-range transport. Here, we show that the SPIRE-type actin nucleators (predominantly SPIRE1) are Rab27a effectors that co-operate with formin-1 to generate actin tracks required for myosin-Va-dependent transport in melanocytes. Thus, in addition to melanophilin/myosin-Va, Rab27a can recruit SPIREs to melanosomes, thereby integrating motor and track assembly activity at the organelle membrane. Based on this, we suggest a model in which organelles and force generators (motors and track assemblers) are linked, forming an organelle-based, cell-wide network that allows their collective activity to rapidly disperse the population of organelles long-distance throughout the cytoplasm.

Date: 2020
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DOI: 10.1038/s41467-020-17212-6

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