Structural snapshots of human pre-60S ribosomal particles before and after nuclear export

Xiaomeng Liang, Mei-Qing Zuo, Yunyang Zhang, Ningning Li, Chengying Ma, Meng-Qiu Dong and Ning Gao ()
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Xiaomeng Liang: Tsinghua University
Mei-Qing Zuo: China Agricultural University
Yunyang Zhang: Peking University
Ningning Li: Peking University
Chengying Ma: Peking University
Meng-Qiu Dong: National Institute of Biological Sciences
Ning Gao: Peking University

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract Ribosome biogenesis is an elaborate and energetically expensive program that involve two hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one central step which also serves as an internal structural checkpoint to ensure the proper completion of nuclear assembly events. Here we present four structures of human pre-60S particles isolated through a nuclear export factor NMD3, representing assembly stages immediately before and after nuclear export. These structures reveal locations of a dozen of human factors, including an uncharacterized factor TMA16 localized between the 5S RNA and the P0 stalk. Comparison of these structures shows a progressive maturation for the functional regions, such as peptidyl transferase centre and peptide exit tunnel, and illustrate a sequence of factor-assisted rRNA maturation events. These data facilitate our understanding of the global conservation of ribosome assembly in eukaryotes and species-specific features of human assembly factors.

Date: 2020
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DOI: 10.1038/s41467-020-17237-x

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