Raman image-activated cell sorting

Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki and Keisuke Goda ()
Additional contact information
Nao Nitta: The University of Tokyo
Takanori Iino: The University of Tokyo
Akihiro Isozaki: The University of Tokyo
Mai Yamagishi: The University of Tokyo
Yasutaka Kitahama: The University of Tokyo
Shinya Sakuma: Nagoya University
Yuta Suzuki: The University of Tokyo
Hiroshi Tezuka: The University of Tokyo
Minoru Oikawa: Kochi University
Fumihito Arai: Nagoya University
Takuya Asai: The University of Tokyo
Dinghuan Deng: The University of Tokyo
Hideya Fukuzawa: Kyoto University
Misa Hase: The University of Tokyo
Tomohisa Hasunuma: Kobe University
Takeshi Hayakawa: Chuo University
Kei Hiraki: The University of Tokyo
Kotaro Hiramatsu: The University of Tokyo
Yu Hoshino: Kyushu University
Mary Inaba: The University of Tokyo
Yuki Inoue: The University of Tokyo
Takuro Ito: The University of Tokyo
Masataka Kajikawa: Kyoto University
Hiroshi Karakawa: The University of Tokyo
Yusuke Kasai: Nagoya University
Yuichi Kato: Kobe University
Hirofumi Kobayashi: The University of Tokyo
Cheng Lei: The University of Tokyo
Satoshi Matsusaka: University of Tsukuba
Hideharu Mikami: The University of Tokyo
Atsuhiro Nakagawa: Tohoku University
Keiji Numata: RIKEN Center for Sustainable Resource Science
Tadataka Ota: The University of Tokyo
Takeichiro Sekiya: The University of Tokyo
Kiyotaka Shiba: Cancer Institute of Japanese Foundation for Cancer Research
Yoshitaka Shirasaki: The University of Tokyo
Nobutake Suzuki: The University of Tokyo
Shunji Tanaka: The University of Tokyo
Shunnosuke Ueno: The University of Tokyo
Hiroshi Watarai: The University of Tokyo
Takashi Yamano: Kyoto University
Masayuki Yazawa: Columbia University
Yusuke Yonamine: Hokkaido University
Dino Carlo: The University of Tokyo
Yoichiroh Hosokawa: Nara Institute of Science and Technology
Sotaro Uemura: The University of Tokyo
Takeaki Sugimura: The University of Tokyo
Yasuyuki Ozeki: The University of Tokyo
Keisuke Goda: The University of Tokyo

Nature Communications, 2020, vol. 11, issue 1, 1-16

Abstract: Abstract The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.

Date: 2020
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DOI: 10.1038/s41467-020-17285-3

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