Detection of the thietane precursor in the UVA formation of the DNA 6-4 photoadduct

Luis A. Ortiz-Rodríguez, Christian Reichardt, Sean J. Hoehn, Steffen Jockusch and Carlos E. Crespo-Hernández ()
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Luis A. Ortiz-Rodríguez: Case Western Reserve University
Christian Reichardt: Case Western Reserve University
Sean J. Hoehn: Case Western Reserve University
Steffen Jockusch: Columbia University
Carlos E. Crespo-Hernández: Case Western Reserve University

Nature Communications, 2020, vol. 11, issue 1, 1-9

Abstract: Abstract Notwithstanding the central biological role of the (6-4) photoadduct in the induction of skin cancer by sunlight, crucial mechanistic details about its formation have evaded characterization despite efforts spanning more than half a century. 4-Thiothymidine (4tT) has been widely used as an important model system to study its mechanism of formation, but the excited-state precursor, the intermediate species, and the time scale leading to the formation of the (6-4) photoadduct have remained elusive. Herein, steady-state and time-resolved spectroscopic techniques are combined with new and reported quantum-chemical calculations to demonstrate the excited state leading to the formation of the thietane intermediate, its rate, and the formation of the (6-4) photoadduct using the 5’-TT(4tT)T(4tT)TT-3’ DNA oligonucleotide. Efficient, sub-1 ps intersystem crossing leads to the population of a triplet minimum of the thietane intermediate in as short as 3 ps, which intersystem crosses to its ground state and rearranges to form the (6-4) photoadduct.

Date: 2020
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DOI: 10.1038/s41467-020-17333-y

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