In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation

Thomas E. Hall (), Nick Martel, Nicholas Ariotti, Zherui Xiong, Harriet P. Lo, Charles Ferguson, James Rae, Ye-Wheen Lim and Robert G. Parton ()
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Thomas E. Hall: University of Queensland
Nick Martel: University of Queensland
Nicholas Ariotti: University of Queensland
Zherui Xiong: University of Queensland
Harriet P. Lo: University of Queensland
Charles Ferguson: University of Queensland
James Rae: University of Queensland
Ye-Wheen Lim: University of Queensland
Robert G. Parton: University of Queensland

Nature Communications, 2020, vol. 11, issue 1, 1-19

Abstract: Abstract The skeletal muscle T-tubule is a specialized membrane domain essential for coordinated muscle contraction. However, in the absence of genetically tractable systems the mechanisms involved in T-tubule formation are unknown. Here, we use the optically transparent and genetically tractable zebrafish system to probe T-tubule development in vivo. By combining live imaging of transgenic markers with three-dimensional electron microscopy, we derive a four-dimensional quantitative model for T-tubule formation. To elucidate the mechanisms involved in T-tubule formation in vivo, we develop a quantitative screen for proteins that associate with and modulate early T-tubule formation, including an overexpression screen of the entire zebrafish Rab protein family. We propose an endocytic capture model involving firstly, formation of dynamic endocytic tubules at transient nucleation sites on the sarcolemma, secondly, stabilization by myofibrils/sarcoplasmic reticulum and finally, delivery of membrane from the recycling endosome and Golgi complex.

Date: 2020
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DOI: 10.1038/s41467-020-17486-w

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