Light microscopy of proteins in their ultrastructural context

Ons M’Saad and Joerg Bewersdorf ()
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Ons M’Saad: Yale School of Medicine
Joerg Bewersdorf: Yale School of Medicine

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of the cell is a major challenge in fluorescence microscopy. We report the discovery of a new principle for an optical contrast equivalent to electron microscopy (EM) which reveals the ultrastructural context of the cells with a conventional confocal microscope. By decrowding the intracellular space through 13 to 21-fold physical expansion while simultaneously retaining the proteins, bulk (pan) labeling of the proteome resolves local protein densities and reveals the cellular nanoarchitecture by standard light microscopy.

Date: 2020
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DOI: 10.1038/s41467-020-17523-8

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