Cell-surface receptors enable perception of extracellular cytokinins

Ioanna Antoniadi, Ondřej Novák, Zuzana Gelová, Alexander Johnson, Ondřej Plíhal, Radim Simerský, Václav Mik, Thomas Vain, Eduardo Mateo-Bonmatí, Michal Karady, Markéta Pernisová, Lenka Plačková, Korawit Opassathian, Jan Hejátko, Stéphanie Robert, Jiří Friml, Karel Doležal, Karin Ljung () and Colin Turnbull ()
Additional contact information
Ioanna Antoniadi: Department of Life Sciences, Imperial College London
Ondřej Novák: Swedish University of Agricultural Sciences
Zuzana Gelová: Institute of Science and Technology Austria
Alexander Johnson: Institute of Science and Technology Austria
Ondřej Plíhal: Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
Radim Simerský: Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
Václav Mik: Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
Thomas Vain: Swedish University of Agricultural Sciences
Eduardo Mateo-Bonmatí: Swedish University of Agricultural Sciences
Michal Karady: Swedish University of Agricultural Sciences
Markéta Pernisová: Faculty of Science, Masaryk University
Lenka Plačková: Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
Korawit Opassathian: Department of Life Sciences, Imperial College London
Jan Hejátko: Faculty of Science, Masaryk University
Stéphanie Robert: Swedish University of Agricultural Sciences
Jiří Friml: Institute of Science and Technology Austria
Karel Doležal: Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
Karin Ljung: Swedish University of Agricultural Sciences
Colin Turnbull: Department of Life Sciences, Imperial College London

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-17700-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17700-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-17700-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().