Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans

Gui-Ping Wen, Linling He, Zi-Min Tang, Si-Ling Wang, Xu Zhang, Yuan-Zhi Chen, Xiaohe Lin, Chang Liu, Jia-Xin Chen, Dong Ying, Zi-Hao Chen, Ying-Bin Wang, Wen-Xin Luo, Shou-Jie Huang, Shao-Wei Li, Jun Zhang, Zi-Zheng Zheng (), Jiang Zhu () and Ning-Shao Xia ()
Additional contact information
Gui-Ping Wen: Xiamen University
Linling He: The Scripps Research Institute
Zi-Min Tang: Xiamen University
Si-Ling Wang: Xiamen University
Xu Zhang: Xiamen University
Yuan-Zhi Chen: Xiamen University
Xiaohe Lin: The Scripps Research Institute
Chang Liu: Xiamen University
Jia-Xin Chen: Xiamen University
Dong Ying: Xiamen University
Zi-Hao Chen: Xiamen University
Ying-Bin Wang: Xiamen University
Wen-Xin Luo: Xiamen University
Shou-Jie Huang: Xiamen University
Shao-Wei Li: Xiamen University
Jun Zhang: Xiamen University
Zi-Zheng Zheng: Xiamen University
Jiang Zhu: The Scripps Research Institute
Ning-Shao Xia: Xiamen University

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract Efficacy evaluation through human trials is crucial for advancing a vaccine candidate to clinics. Next-generation sequencing (NGS) can be used to quantify B cell repertoire response and trace antibody lineages during vaccination. Here, we demonstrate this application with a case study of Hecolin®, the licensed vaccine for hepatitis E virus (HEV). Four subjects are administered the vaccine following a standard three-dose schedule. Vaccine-induced antibodies exhibit a high degree of clonal diversity, recognize five conformational antigenic sites of the genotype 1 HEV p239 antigen, and cross-react with other genotypes. Unbiased repertoire sequencing is performed for seven time points over six months of vaccination, with maturation pathways characterize for a set of vaccine-induced antibodies. In addition to dynamic repertoire profiles, NGS analysis reveals differential patterns of HEV-specific antibody lineages and highlights the necessity of the long vaccine boost. Together, our study presents a quantitative strategy for vaccine evaluation in small-scale human studies.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-020-17737-w Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17737-w

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-17737-w

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().