Widespread protein lysine acetylation in gut microbiome and its alterations in patients with Crohn’s disease

Xu Zhang, Zhibin Ning, Janice Mayne, Yidai Yang, Shelley A. Deeke, Krystal Walker, Charles L. Farnsworth, Matthew P. Stokes, Jean-François Couture, David Mack, Alain Stintzi () and Daniel Figeys ()
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Xu Zhang: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa
Zhibin Ning: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa
Janice Mayne: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa
Yidai Yang: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa
Shelley A. Deeke: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa
Krystal Walker: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa
Charles L. Farnsworth: Cell Signaling Technology Inc.
Matthew P. Stokes: Cell Signaling Technology Inc.
Jean-François Couture: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa
David Mack: Department of Pediatrics, Faculty of Medicine, University of Ottawa and Children’s Hospital of Eastern Ontario Inflammatory Bowel Disease Centre and Research Institute
Alain Stintzi: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa
Daniel Figeys: Shanghai Institute of Materia Medica-University of Ottawa Joint Research Center in Systems and Personalized Pharmacology, University of Ottawa

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract Lysine acetylation (Kac), an abundant post-translational modification (PTM) in prokaryotes, regulates various microbial metabolic pathways. However, no studies have examined protein Kac at the microbiome level, and it remains unknown whether Kac level is altered in patient microbiomes. Herein, we use a peptide immuno-affinity enrichment strategy coupled with mass spectrometry to characterize protein Kac in the microbiome, which successfully identifies 35,200 Kac peptides from microbial or human proteins in gut microbiome samples. We demonstrate that Kac is widely distributed in gut microbial metabolic pathways, including anaerobic fermentation to generate short-chain fatty acids. Applying to the analyses of microbiomes of patients with Crohn’s disease identifies 52 host and 136 microbial protein Kac sites that are differentially abundant in disease versus controls. This microbiome-wide acetylomic approach aids in advancing functional microbiome research.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17916-9

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DOI: 10.1038/s41467-020-17916-9

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