Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum

Karolina Kubiasová, Juan Carlos Montesinos, Olga Šamajová, Jaroslav Nisler, Václav Mik, Hana Semerádová, Lucie Plíhalová, Ondřej Novák, Peter Marhavý, Nicola Cavallari, David Zalabák, Karel Berka, Karel Doležal, Petr Galuszka, Jozef Šamaj, Miroslav Strnad, Eva Benková (), Ondřej Plíhal () and Lukáš Spíchal ()
Additional contact information
Karolina Kubiasová: Palacký University
Juan Carlos Montesinos: Institute of Science and Technology (IST)
Olga Šamajová: Palacký University
Jaroslav Nisler: Palacký University
Václav Mik: Palacký University
Hana Semerádová: Institute of Science and Technology (IST)
Lucie Plíhalová: Palacký University
Ondřej Novák: Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
Peter Marhavý: Institute of Science and Technology (IST)
Nicola Cavallari: Institute of Science and Technology (IST)
David Zalabák: Palacký University
Karel Berka: Palacký University
Karel Doležal: Palacký University
Jozef Šamaj: Palacký University
Miroslav Strnad: Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
Eva Benková: Institute of Science and Technology (IST)
Ondřej Plíhal: Palacký University
Lukáš Spíchal: Palacký University

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER.

Date: 2020
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DOI: 10.1038/s41467-020-17949-0

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