NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing

Lingfei Lu, Benhao Li, Suwan Ding, Yong Fan (), Shangfeng Wang, Caixia Sun, Mengyao Zhao, Chun-Xia Zhao and Fan Zhang ()
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Lingfei Lu: Fudan University
Benhao Li: Fudan University
Suwan Ding: Fudan University
Yong Fan: Fudan University
Shangfeng Wang: Fudan University
Caixia Sun: Fudan University
Mengyao Zhao: Fudan University
Chun-Xia Zhao: The University of Queensland
Fan Zhang: Fudan University

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Bioluminescence imaging has been widely used in life sciences and biomedical applications. However, conventional bioluminescence imaging usually operates in the visible region, which hampers the high-performance in vivo optical imaging due to the strong tissue absorption and scattering. To address this challenge, here we present bioluminescence probes (BPs) with emission in the second near infrared (NIR-II) region at 1029 nm by employing bioluminescence resonance energy transfer (BRET) and two-step fluorescence resonance energy transfer (FRET) with a specially designed cyanine dye FD-1029. The biocompatible NIR-II-BPs are successfully applied to vessels and lymphatics imaging in mice, which gives ~5 times higher signal-to-noise ratios and ~1.5 times higher spatial resolution than those obtained by NIR-II fluorescence imaging and conventional bioluminescence imaging. Their capability of multiplexed imaging is also well displayed. Taking advantage of the ATP-responding character, the NIR-II-BPs are able to recognize tumor metastasis with a high tumor-to-normal tissue ratio at 83.4.

Date: 2020
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DOI: 10.1038/s41467-020-18051-1

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