Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila

Bumsik Cho, Sang-Ho Yoon, Daewon Lee, Ferdinand Koranteng, Sudhir Gopal Tattikota, Nuri Cha, Mingyu Shin, Hobin Do, Yanhui Hu, Sue Young Oh, Daehan Lee, A. Vipin Menon, Seok Jun Moon, Norbert Perrimon, Jin-Wu Nam () and Jiwon Shim ()
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Bumsik Cho: Hanyang University
Sang-Ho Yoon: Hanyang University
Daewon Lee: Hanyang University
Ferdinand Koranteng: Hanyang University
Sudhir Gopal Tattikota: Harvard Medical School
Nuri Cha: Hanyang University
Mingyu Shin: Hanyang University
Hobin Do: Hanyang University
Yanhui Hu: Harvard Medical School
Sue Young Oh: Yonsei University, College of Dentistry
Daehan Lee: Hanyang University
A. Vipin Menon: Hanyang University
Seok Jun Moon: Yonsei University, College of Dentistry
Norbert Perrimon: Harvard Medical School
Jin-Wu Nam: Hanyang University
Jiwon Shim: Hanyang University

Nature Communications, 2020, vol. 11, issue 1, 1-18

Abstract: Abstract The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution.

Date: 2020
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DOI: 10.1038/s41467-020-18135-y

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