Fast and multiplexed superresolution imaging with DNA-PAINT-ERS

Fehmi Civitci, Julia Shangguan, Ting Zheng, Kai Tao, Matthew Rames, John Kenison, Ying Zhang, Lei Wu, Carey Phelps, Sadik Esener and Xiaolin Nan ()
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Fehmi Civitci: Oregon Health and Science University
Julia Shangguan: Oregon Health and Science University
Ting Zheng: Oregon Health and Science University
Kai Tao: Oregon Health and Science University
Matthew Rames: Oregon Health and Science University
John Kenison: Oregon Health and Science University
Ying Zhang: Oregon Health and Science University
Lei Wu: Oregon Health and Science University
Carey Phelps: Oregon Health and Science University
Sadik Esener: Oregon Health and Science University
Xiaolin Nan: Oregon Health and Science University

Nature Communications, 2020, vol. 11, issue 1, 1-8

Abstract: Abstract DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.

Date: 2020
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DOI: 10.1038/s41467-020-18181-6

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