Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet

Song Liu, Shaochun Yuan (), Xiaoman Gao, Xin Tao, Wenjuan Yu, Xu Li, Shangwu Chen and Anlong Xu ()
Additional contact information
Song Liu: School of Life Sciences, Sun Yat-sen University
Shaochun Yuan: School of Life Sciences, Sun Yat-sen University
Xiaoman Gao: School of Life Sciences, Sun Yat-sen University
Xin Tao: School of Life Sciences, Sun Yat-sen University
Wenjuan Yu: School of Life Sciences, Sun Yat-sen University
Xu Li: School of Life Sciences, Sun Yat-sen University
Shangwu Chen: School of Life Sciences, Sun Yat-sen University
Anlong Xu: School of Life Sciences, Sun Yat-sen University

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract The discovery of ancestral RAG transposons in early deuterostomia reveals the origin of vertebrate V(D)J recombination. Here, we analyze the functional regulation of a RAG transposon, ProtoRAG, in lancelet. We find that a specific interaction between the cis-acting element within the TIR sequences of ProtoRAG and a trans-acting factor, lancelet YY1-like (bbYY1), is important for the transcriptional regulation of lancelet RAG-like genes (bbRAG1L and bbRAG2L). Mechanistically, bbYY1 suppresses the transposition of ProtoRAG; meanwhile, bbYY1 promotes host DNA rejoins (HDJ) and TIR-TIR joints (TTJ) after TIR-dependent excision by facilitating the binding of bbRAG1L/2 L to TIR-containing DNA, and by interacting with the bbRAG1L/2 L complex. Our data thus suggest that bbYY1 has dual functions in fine-tuning the activity of ProtoRAG and maintaining the genome stability of the host.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-18261-7 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18261-7

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-18261-7

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().