In vivo localization of chronically implanted electrodes and optic fibers in mice

Bálint Király, Diána Balázsfi, Ildikó Horváth, Nicola Solari, Katalin Sviatkó, Katalin Lengyel, Eszter Birtalan, Magor Babos, Gergő Bagaméry, Domokos Máthé, Krisztián Szigeti and Balázs Hangya ()
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Bálint Király: Lendület Laboratory of Systems Neuroscience, Institute of Experimental Medicine
Diána Balázsfi: Lendület Laboratory of Systems Neuroscience, Institute of Experimental Medicine
Ildikó Horváth: Semmelweis University
Nicola Solari: Lendület Laboratory of Systems Neuroscience, Institute of Experimental Medicine
Katalin Sviatkó: Lendület Laboratory of Systems Neuroscience, Institute of Experimental Medicine
Katalin Lengyel: Lendület Laboratory of Systems Neuroscience, Institute of Experimental Medicine
Eszter Birtalan: Lendület Laboratory of Systems Neuroscience, Institute of Experimental Medicine
Magor Babos: Mediso Medical Imaging Systems Ltd.
Gergő Bagaméry: Mediso Medical Imaging Systems Ltd.
Domokos Máthé: Semmelweis University
Krisztián Szigeti: Semmelweis University
Balázs Hangya: Lendület Laboratory of Systems Neuroscience, Institute of Experimental Medicine

Nature Communications, 2020, vol. 11, issue 1, 1-17

Abstract: Abstract Electrophysiology provides a direct readout of neuronal activity at a temporal precision only limited by the sampling rate. However, interrogating deep brain structures, implanting multiple targets or aiming at unusual angles still poses significant challenges for operators, and errors are only discovered by post-hoc histological reconstruction. Here, we propose a method combining the high-resolution information about bone landmarks provided by micro-CT scanning with the soft tissue contrast of the MRI, which allowed us to precisely localize electrodes and optic fibers in mice in vivo. This enables arbitrating the success of implantation directly after surgery with a precision comparable to gold standard histology. Adjustment of the recording depth with micro-drives or early termination of unsuccessful experiments saves many working hours, and fast 3-dimensional feedback helps surgeons avoid systematic errors. Increased aiming precision enables more precise targeting of small or deep brain nuclei and multiple targeting of specific cortical or hippocampal layers.

Date: 2020
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DOI: 10.1038/s41467-020-18472-y

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