Mechanism of self/nonself-discrimination in Brassica self-incompatibility

Kohji Murase, Yoshitaka Moriwaki (), Tomoyuki Mori, Xiao Liu, Chiho Masaka, Yoshinobu Takada, Ryoko Maesaki, Masaki Mishima, Sota Fujii, Yoshinori Hirano, Zen Kawabe, Koji Nagata, Tohru Terada, Go Suzuki, Masao Watanabe, Kentaro Shimizu, Toshio Hakoshima () and Seiji Takayama ()
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Kohji Murase: The University of Tokyo
Yoshitaka Moriwaki: The University of Tokyo
Tomoyuki Mori: Nara Institute of Science and Technology
Xiao Liu: Nara Institute of Science and Technology
Chiho Masaka: Nara Institute of Science and Technology
Yoshinobu Takada: Tohoku University
Ryoko Maesaki: Tokyo Metropolitan University
Masaki Mishima: Tokyo Metropolitan University
Sota Fujii: The University of Tokyo
Yoshinori Hirano: Nara Institute of Science and Technology
Zen Kawabe: The University of Tokyo
Koji Nagata: The University of Tokyo
Tohru Terada: The University of Tokyo
Go Suzuki: Osaka Kyoiku University
Masao Watanabe: Tohoku University
Kentaro Shimizu: The University of Tokyo
Toshio Hakoshima: Nara Institute of Science and Technology
Seiji Takayama: The University of Tokyo

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Self-incompatibility (SI) is a breeding system that promotes cross-fertilization. In Brassica, pollen rejection is induced by a haplotype-specific interaction between pistil determinant SRK (S receptor kinase) and pollen determinant SP11 (S-locus Protein 11, also named SCR) from the S-locus. Although the structure of the B. rapa S9-SRK ectodomain (eSRK) and S9-SP11 complex has been determined, it remains unclear how SRK discriminates self- and nonself-SP11. Here, we uncover the detailed mechanism of self/nonself-discrimination in Brassica SI by determining the S8-eSRK–S8-SP11 crystal structure and performing molecular dynamics (MD) simulations. Comprehensive binding analysis of eSRK and SP11 structures reveals that the binding free energies are most stable for cognate eSRK–SP11 combinations. Residue-based contribution analysis suggests that the modes of eSRK–SP11 interactions differ between intra- and inter-subgroup (a group of phylogenetically neighboring haplotypes) combinations. Our data establish a model of self/nonself-discrimination in Brassica SI.

Date: 2020
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DOI: 10.1038/s41467-020-18698-w

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